LOCUS       pAAV-TetOne-Luc Control Vector           6417 bp ds-DNA    circular SYN 13-NOV-2014
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat No. 634310, 634311
SOURCE      synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    1..141
                     /label="5' ITR"
     polyA_signal    148..282
                     /label="SV40 polyA signal"
     CDS             complement(283..1029)
                     /label="Tet-On 3G"
     promoter        complement(1048..1558)
                     /label="hPGK promoter"
     misc_feature    1578..1731
                     /label="Transcription blocker"
                     /note="Transcription blocker;RNA polymerase II 
                     transcriptional pause signal from the human alpha-2 globin 
                     gene combined with a synthetic polyA signal"
     promoter        1738..2102
                     /label="TRE3GS promoter"
     CDS             2112..3764
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    3791..3925
                     /label="SV40 polyA signal"
     misc_feature    3935..4075
                     /label="3' ITR"
     rep_origin      complement(4493..5081)
                     /label="ori"
                     /note="ori;high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5252..6112)
                     /label="AmpR"
                     /note="beta-lactamase, confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(6113..6217)
                     /label="AmpR promoter"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tagatctaac ttgtttattg cagcttataa tggttacaaa
      181 taaggcaata gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt
      241 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcttacccgg ggagcatgtc
      301 aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc
      361 atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc cgccgctttc
      421 gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg
      481 ttcggctccc tgccggtcga acagctcaat tgcttgtttc agaagtgggg gcatagaatc
      541 ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca
      601 gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc
      661 ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg
      721 ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggtatgactt
      781 ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcagg agtgggtatg
      841 atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg
      901 ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc
      961 ttcgataccg actccattga gtaattccag agcagagttt atgactttgc tcttgtccag
     1021 tctagacatg gtaattcgat gatccccctg gggagagagg tcggtgattc ggtcaacgag
     1081 ggagccgact gccgacgtgc gctccggagg cttgcagaat gcggaacacc gcgcgggcag
     1141 gaacagggcc cacactaccg ccccacaccc cgcctcccgc accgcccctt cccggccgct
     1201 gctctcggcg cgccctgctg agcagccgct attggccaca gcccatcgcg gtcggcgcgc
     1261 tgccattgct ccctggcgct gtccgtctgc gagggtacta gtgagacgtg cggcttccgt
     1321 ttgtcacgtc cggcacgccg cgaaccgcaa ggaaccttcc cgacttaggg gcggagcagg
     1381 aagcgtcgcc ggggggccca caagggtagc ggcgaagatc cgggtgacgc tgcgaacgga
     1441 cgtgaagaat gtgcgagacc cagggtcggc gccgctgcgt ttcccggaac cacgcccaga
     1501 gcagccgcgt ccctgcgcaa acccagggct gccttggaaa aggcgcaacc ccaaccccgt
     1561 ggaattatca cctcgagaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     1621 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     1681 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tctcgagttt
     1741 actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg
     1801 tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag
     1861 tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt
     1921 actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg
     1981 tataagcttt gcttatgtaa accagggcgc ctataaaaga gtgctgattt tttgagtaaa
     2041 cttcaattcc acaacacttt tgtcttatac caactttccg taccacttcc taccctcgta
     2101 aagaattcac catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc
     2161 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
     2221 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact
     2281 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
     2341 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
     2401 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
     2461 gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt
     2521 tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg
     2581 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
     2641 atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga
     2701 actcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg
     2761 tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
     2821 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
     2881 tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ttacgatccc
     2941 ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca ttcttcgcca
     3001 aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct tctgggggcg
     3061 cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt ccagggatac
     3121 gacaaggata tgggctcact gagactacat cagctattct gattacaccc gagggggatg
     3181 ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg
     3241 ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga ggacctatga
     3301 ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat
     3361 ggctacattc tggagacata gcttactggg acgaagacga acacttcttc atagttgacc
     3421 gcttgaagtc tttaattaaa tacaaaggat accaggtggc ccccgctgaa ttggagtcga
     3481 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg
     3541 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag
     3601 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg
     3661 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
     3721 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaagcggcc gcacgcgtgg
     3781 atccaccggt gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
     3841 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
     3901 cattataagc tgcaataaac aagttgatat cgataggaac ccctagtgat ggagttggcc
     3961 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4021 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggcaatt
     4081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4261 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6241 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     6301 ggccctttcg tctcgcgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     6361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
