LOCUS       T-Vector pMD20 Vector (Linear)     2736 bp DNA     linear   SYN 29-OCT-2021
COMMENT	    Created by Takara Bio Inc. https://www.takarabio.com
COMMENT	    Cat No. 3270
FEATURES             Location/Qualifiers
     CDS             1..193
                     /label="lacZ-alpha"
     misc_feature    1..30
                     /label="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(31..47)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             625..1485
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      1656..2244
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2568..2598
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2606..2622
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2629..2646
                     /label="M13"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2642..2736
                     /label="lacZ-alpha"
     misc_feature    2686..2736
                     /label="MCS"
                     /note="multiple cloning site"
ORIGIN
        1 atcggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
       61 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      181 cgaatggccc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      241 tagtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
      301 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      361 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      421 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      481 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      541 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      601 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      661 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      721 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      781 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      841 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      901 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      961 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1021 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1081 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1141 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1201 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1261 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1321 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1381 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1441 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1621 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1801 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2041 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2221 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2281 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2341 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2401 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2461 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2521 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2581 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2641 tatgaccatg attacgccaa gctatttagg tgacactata ggggaaagct tgcatgcctg
     2701 caggtcgact ctagaggatc tactagtcat atggat
//
