LOCUS       Postive Control Template (Fluc)     4574 bp DNA     circular SYN 29-MAR-2022
COMMENT     Created by Takara Bio, Inc. http://www.takarabio.com
COMMENT     Cat No. 6141, 6144
FEATURES             Location/Qualifiers
     CDS             193..987
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
     rep_origin      1312..1900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2001..2380
                     /label=CMV enhancer
     promoter        2381..2584
                     /label=CMV promoter
     promoter        2629..2645
                     /label=T7 promoter
     5'UTR           2646..2699
		     /label=5'UTR	
     CDS             2700..4352
                     /label=Fluc
     3'UTR           4353..4465
                     /label=3'UTR
     polyA_site      4466..4570
                     /label=Poly (A)
ORIGIN
        1 ctcgagtcga cctaggatcc cttctactgg gcggttttat ggacagcaag cgaaccggaa
       61 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
      121 ttcttgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
      181 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
      241 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
      301 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
      361 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
      421 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
      481 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
      541 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
      601 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
      661 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
      721 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
      781 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
      841 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
      901 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
      961 tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat
     1021 tttctcctta cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa
     1081 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1141 gagacaataa ccctgataaa tgcttcaata atagcacgtg ctaaaacttc atttttaatt
     1201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1441 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1681 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttcttgctgc ttcgcgatgt
     1981 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     2041 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2101 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2161 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2221 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2281 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2341 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2401 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2461 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2521 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2581 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     2641 ctataaggaa taaactagta ttcttctggt ccccacagac tcagagagaa cccgccacca
     2701 tggaggacgc caagaacatc aagaagggcc ccgccccctt ctaccccctg gaggacggca
     2761 ccgccggcga gcagctgcac aaggccatga agcggtacgc cctggtgccc ggcaccatcg
     2821 ccttcaccga cgcccacatc gaggtggaca tcacctacgc cgagtacttc gagatgagcg
     2881 tgcggctggc cgaggccatg aagcggtacg gcctgaacac caaccaccgg atcgtggtgt
     2941 gcagcgagaa cagcctgcag ttcttcatgc ccgtgctggg cgccctgttc atcggcgtgg
     3001 ccgtggcccc cgccaacgac atctacaacg agcgggagct gctgaacagc atgggcatca
     3061 gccagcccac cgtggtgttc gtgagcaaga agggcctgca gaagatcctg aacgtgcaga
     3121 agaagctgcc catcatccag aagatcatca tcatggacag caagaccgac taccagggct
     3181 tccagagcat gtacaccttc gtgaccagcc acctgccccc cggcttcaac gagtacgact
     3241 tcgtgcccga gagcttcgac cgggacaaga ccatcgccct gatcatgaac agcagcggca
     3301 gcaccggcct gcccaagggc gtggccctgc cccaccggac cgcctgcgtg cggttcagcc
     3361 acgcccggga ccccatcttc ggcaaccaga tcatccccga caccgccatc ctgagcgtgg
     3421 tgcccttcca ccacggcttc ggcatgttca ccaccctggg ctacctgatc tgcggcttcc
     3481 gggtggtgct gatgtaccgg ttcgaggagg agctgttcct gcggagcctg caggactaca
     3541 agatccagag cgccctgctg gtgcccaccc tgttcagctt cttcgccaag agcaccctga
     3601 tcgacaagta cgacctgagc aacctgcacg agatcgccag cggcggcgcc cccctgagca
     3661 aggaggtggg cgaggccgtg gccaagcggt tccacctgcc cggcatccgg cagggctacg
     3721 gcctgaccga gaccaccagc gccatcctga tcacccccga gggcgacgac aagcccggcg
     3781 ccgtgggcaa ggtggtgccc ttcttcgagg ccaaggtggt ggacctggac accggcaaga
     3841 ccctgggcgt gaaccagcgg ggcgagctgt gcgtgcgggg ccccatgatc atgagcggct
     3901 acgtgaacaa ccccgaggcc accaacgccc tgatcgacaa ggacggctgg ctgcacagcg
     3961 gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc
     4021 tgatcaagta caagggctac caggtggccc ccgccgagct ggagagcatc ctgctgcagc
     4081 accccaacat cttcgacgcc ggcgtggccg gcctgcccga cgacgacgcc ggcgagctgc
     4141 ccgccgccgt ggtggtgctg gagcacggca agaccatgac cgagaaggag atcgtggact
     4201 acgtggccag ccaggtgacc accgccaaga agctgcgggg cggcgtggtg ttcgtggacg
     4261 aggtgcccaa gggcctgacc ggcaagctgg acgcccggaa gatccgggag atcctgatca
     4321 aggccaagaa gggcggcaag atcgccgtgt gatgagctgg agcctcggtg gcctagcttc
     4381 ttgccccttg ggcctccccc cagcccctcc tccccttcct gcacccgtac ccccgtggtc
     4441 tttgaataaa gtctgagtgg gcggcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4501 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4561 aaaaaaaaaa gctt
//
