LOCUS       Linearized pRACE           2656 bp ds-DNA    linear   SYN 12-FEB-2014
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat No. 634858, 634860
SOURCE      synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    1
                     /label="In-Fusion Cloning Site"
                     /note="linearized pRACE In-Fusion cloning site"
     misc_feature    1..15
                     /label="In-Fusion Homology to be Added to GSP"
     primer_bind     complement(19..35)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    43..59
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(67..97)
                     /label="lac promoter"
     rep_origin      complement(421..1009)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1180..2040)
                     /label="AmpR"
                     /note="beta-lactamase, confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(2041..2145)
                     /note="AmpR promoter"
     primer_bind     2619..2635
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2642..2656
                     /label="In-Fusion Homology to UPM"
ORIGIN
        1 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
       61 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2161 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2221 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     2281 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     2341 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     2401 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     2461 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     2521 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     2581 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     2641 cctaatacga ctcact
//
