LOCUS       Linearized_Templ        2921 bp DNA     linear   UNA 08-FEB-2024
DEFINITION  natural linear DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 8, 2024 from SnapGene 6.2.1
            https://www.snapgene.com
COMMENT     Created by Takara Bio, Inc. http://www.takarabio.com
            Cat. No. 6141, 6143
FEATURES             Location/Qualifiers
     source          1..2921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     3'UTR           1..113
                     /label=3' UTR
     misc_feature    1..15
                     /label=Downstream In-Fusion Cloning site
     primer_bind     11..30
                     /label=Poly (A) forward primer
     polyA_site      114..218
                     /label=Poly (A)
     primer_bind     complement(304..323)
                     /label=Poly (A) reverse primer
     CDS             415..1209
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      1534..2122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        2223..2602
                     /label=CMV enhancer
     promoter        2603..2806
                     /label=CMV promoter
     promoter        2851..2867
                     /label=T7 promoter
     5'UTR           2868..2921
                     /label=5' UTR
     misc_feature    2907..2921
                     /label=Upstream In-Fusion Cloning site
ORIGIN
        1 tgagctggag cctcggtggc ctagcttctt gccccttggg cctcccccca gcccctcctc
       61 cccttcctgc acccgtaccc ccgtggtctt tgaataaagt ctgagtgggc ggcaaaaaaa
      121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagc ttctcgagtc gacctaggat
      241 cccttctact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc
      301 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
      361 tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt
      421 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      481 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      541 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
      601 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      661 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      721 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      781 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      841 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      901 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
      961 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1021 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1081 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1141 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1201 ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     1261 tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1381 aatgcttcaa taatagcacg tgctaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1681 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2101 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2161 ttgctggcct tttgctcaca tgttcttgct gcttcgcgat gtacgggcca gatatacgcg
     2221 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2281 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2341 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2401 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2461 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2521 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2581 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2641 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2701 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2761 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     2821 agaacccact gcttactggc ttatcgaaat taatacgact cactataagg aataaactag
     2881 tattcttctg gtccccacag actcagagag aacccgccac c
//
