LOCUS       pTet-On-Advanced   7139 bp    DNA   circular             2-FEB-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631118,631120,630930,631069,631124
FEATURES             Location/Qualifiers
     misc_feature    86..677
                     /label=CMV\IE\Promoter
     misc_feature    775..1521
                     /label=rtTA-Advanced\(Tet-On\Advanced)
     misc_feature    1544..1986
                     /label=fragment\containing\SV40\polyA\signal
     misc_feature    2354..2997
                     /label=pUC\origin
     misc_feature    complement(3144..4004)
                     /label=Amp(R)
     misc_feature    complement(5416..6210)
                     /label=Kan(R)/Neo(R)
     misc_feature    complement(6531..6874)
                     /label=SV40\Promoter
BASE COUNT     1794 a      1847 c      1766 g      1732 t 
ORIGIN
        1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt 
       61 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa 
      121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 
      181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 
      241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 
      301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 
      361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 
      421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 
      481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 
      541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 
      601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 
      661 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 
      721 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccatgtct 
      781 agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg agtcggtatc 
      841 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac 
      901 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg 
      961 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac 
     1021 gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc 
     1081 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag 
     1141 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg 
     1201 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc 
     1261 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc 
     1321 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag 
     1381 tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc 
     1441 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac 
     1501 cttgacatgc tccccgggta actaagtaag gatccagaca tgataagata cattgatgag 
     1561 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 
     1621 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 
     1681 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac 
     1741 ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct ggccggacca 
     1801 cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag cgcccgccgc 
     1861 cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc ggtaaccggc 
     1921 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg 
     1981 aagtatccag ctcgaccaag cttggcgaga ttttcaggag ctaaggaagc taaaatggag 
     2041 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 
     2101 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct gcattaatga 
     2161 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     2221 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     2281 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     2341 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     2401 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     2461 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     2521 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     2581 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     2641 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     2701 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     2761 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     2821 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     2881 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     2941 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     3001 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     3061 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     3121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     3181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     3241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     3301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     3361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     3421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     3481 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     3541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     3601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     3661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     3721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 
     3781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     3841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     3901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     3961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     4021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     4081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     4141 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     4201 cgtcttcact cgaggtcgag ggatccagac atgataagat acattgatga gtttggacaa 
     4261 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 
     4321 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 
     4381 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa 
     4441 tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa 
     4501 cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag 
     4561 acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc 
     4621 tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt 
     4681 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca 
     4741 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg 
     4801 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg 
     4861 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat 
     4921 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta 
     4981 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca 
     5041 caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc 
     5101 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga 
     5161 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc 
     5221 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct 
     5281 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg 
     5341 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga 
     5401 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga 
     5461 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc 
     5521 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg 
     5581 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc 
     5641 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa 
     5701 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc 
     5761 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca 
     5821 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc 
     5881 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca 
     5941 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc 
     6001 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt 
     6061 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca 
     6121 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga 
     6181 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc 
     6241 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt 
     6301 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt 
     6361 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct 
     6421 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca 
     6481 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc 
     6541 tgagtgcttg cggcagcgtg aagctagctt tttgcaaaag cctaggcctc caaaaaagcc 
     6601 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa 
     6661 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat 
     6721 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact 
     6781 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat 
     6841 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac 
     6901 attccacagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 
     6961 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 
     7021 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 
     7081 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcacct 
//
