LOCUS       pRetroQ-DsRed-Monomer-N1   7571 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632507
FEATURES             Location/Qualifiers
     misc_feature    1..727
                     /label=CMV/MSV\5'\LTR
     misc_feature    757..1566
                     /label=Extended\packaging\signal
     misc_feature    4270..4702
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(6349..7209)
                     /label=Amp(R)
     rep_origin      5589..5589
                     /label=pUC\origin
     promoter        2957..3465
                     /label=PGK\Promoter
     CDS             3486..4085
                     /label=Puro(R)
     promoter        1582..2170
                     /label=CMV\IE\Promoter
     misc_feature    2172..2246
                     /label=MCS
     CDS             2260..2937
                     /label=DsRed-Monomer
     misc_feature    2253..2263
                     /label=Kozak\Sequence
     rep_origin      5203..5268
                     /label=SV40\origin
     promoter        4982..5249
                     /label=SV40\Promoter
BASE COUNT     1695 a      2186 c      1988 g      1702 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag 
     2221 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggacaacac cgaggacgtc 
     2281 atcaaggagt tcatgcagtt caaggtgcgc atggagggct ccgtgaacgg ccactacttc 
     2341 gagatcgagg gcgagggcga gggcaagccc tacgagggca cccagaccgc caagctgcag 
     2401 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac 
     2461 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacatgaa gctgtccttc 
     2521 cccgagggct tcacctggga gcgctccatg aacttcgagg acggcggcgt ggtggaggtg 
     2581 cagcaggact cctccctgca ggacggcacc ttcatctaca aggtgaagtt caagggcgtg 
     2641 aacttccccg ccgacggccc cgtaatgcag aagaagactg ccggctggga gccctccacc 
     2701 gagaagctgt acccccagga cggcgtgctg aagggcgaga tctcccacgc cctgaagctg 
     2761 aaggacggcg gccactacac ctgcgacttc aagaccgtgt acaaggccaa gaagcccgtg 
     2821 cagctgcccg gcaaccacta cgtggactcc aagctggaca tcaccaacca caacgaggac 
     2881 tacaccgtgg tggagcagta cgagcacgcc gaggcccgcc actccggctc ccagtagagc 
     2941 ggccgcgact ctagataatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga 
     3001 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc 
     3061 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg 
     3121 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc 
     3181 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct 
     3241 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct 
     3301 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg 
     3361 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc 
     3421 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc 
     3481 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg 
     3541 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc 
     3601 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 
     3661 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 
     3721 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 
     3781 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 
     3841 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 
     3901 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 
     3961 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 
     4021 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg 
     4081 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat 
     4141 cggcgtctcg atcgagatat cagtggtcca ggctctagtt ttgactcaac aatatcacca 
     4201 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa 
     4261 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctactagctt aagtaacgcc 
     4321 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg 
     4381 aacagatgga acagggtcga tcgaccctag agaaccatca gatgtttcca gggtgcccca 
     4441 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct 
     4501 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg 
     4561 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag 
     4621 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac 
     4681 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg 
     4741 accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg 
     4801 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 
     4861 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 
     4921 tgacccagtc acgtagcgat agcggagtgt agatccggct gtggaatgtg tgtcagttag 
     4981 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     5041 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     5101 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     5161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     5221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     5281 gcctaggctt ttgcaaaaag cttactggct taactatgcg gcatcagagc agattgtact 
     5341 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 
     5401 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 
     5461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 
     5521 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     5581 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     5641 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     5701 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     5761 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     5821 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     5881 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     5941 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     6001 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 
     6061 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     6121 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     6181 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     6241 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     6301 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     6361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     6421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     6481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     6541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     6601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     6661 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     6721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     6781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     6841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     6901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     6961 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     7021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     7081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     7141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     7201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     7261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     7321 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 
     7381 aaataggcgt atcacgaggc cctttcgtct tcaagaatta gcttggccat tgcatacgtt 
     7441 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg 
     7501 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 
     7561 atatatggag t 
//
