LOCUS       pIRES2-DsRed-Express2   5265 bp    DNA   circular             6-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 
FEATURES             Location/Qualifiers
     CDS             3161..3955
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     promoter        1..589
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569"
     polyA_signal    2084..2134
                     /label=SV40\early\polyA\signal
                     /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602"
     polyA_signal    4191..4209
                     /label=HSV_TK_polyA\signal
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677"
     promoter        2810..3078
                     /label=SV40\early\Promoter
                     /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546"
     promoter        2698..2733
                     /label=Kan(R)\Promoter
                     /note="Bacterial promoter for expression of Kan R gene; -35 region: 2166-2171; -10 region: 2189-2194; Transcription start point: 2201"
     rep_origin      complement(2181..2636)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      2977..3112
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      4540..5183
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     misc_signal     591..665
                     /label=MCS
                     /note="Multiple Cloning Site"
     misc_feature    666..1250
                     /label=IRES2
     CDS             1254..1928
                     /label=DsRed-Express2
BASE COUNT     1247 a      1422 c      1393 g      1203 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 
      721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 
      781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 
      841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 
      901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 
      961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 
     1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 
     1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 
     1141 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 
     1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggata 
     1261 gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt gcacatggag ggctccgtga 
     1321 acggccacga gttcgagatc gagggcgagg gcgagggcaa gccctacgag ggcacccaga 
     1381 ccgccaagct gcaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc 
     1441 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca 
     1501 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 
     1561 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg caccttcatc taccacgtga 
     1621 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actctgggct 
     1681 gggagccctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca 
     1741 aggcgctgaa gctgaagggc ggcggccact acctggtgga gttcaagtca atctacatgg 
     1801 ccaagaagcc cgtgaagctg cccggctact actacgtgga ctccaagctg gacatcacct 
     1861 cccacaacga ggactacacc gtggtggagc agtacgagcg cgccgaggcc cgccaccacc 
     1921 tgttccagta ggcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt 
     1981 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 
     2041 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     2101 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     2161 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 
     2221 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     2281 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     2341 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     2401 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     2461 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     2521 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     2581 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg 
     2641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     2701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     2761 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 
     2821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
     2881 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     2941 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     3001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     3061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     3121 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     3181 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     3241 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     3301 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 
     3361 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     3421 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     3481 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     3541 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     3601 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     3661 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 
     3721 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     3781 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     3841 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     3901 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     3961 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     4021 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     4081 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 
     4141 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 
     4201 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 
     4261 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 
     4321 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 
     4381 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 
     4441 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     4501 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     4561 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     4621 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     4681 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     4741 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     4801 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     4861 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     4921 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     4981 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     5041 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     5101 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     5161 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     5221 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat 
//
