LOCUS       pIRES2-AcGFP1-Nuc   5417 bp    DNA   circular             1-FEB-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632435
FEATURES             Location/Qualifiers
     misc_feature    1..589
                     /label=CMV\IE\Promoter
     misc_feature    591..661
                     /label=MCS
     misc_feature    666..1250
                     /label=IRES
     misc_feature    1254..1970
                     /label=AcGFP1
     misc_feature    1971..2081
                     /label=Nuclear\Localization\Signal\(NLS)
     misc_feature    2333..2788
                     /label=f1\origin
     misc_feature    2236..2286
                     /label=SV40\early\polyA\signals
     misc_feature    2962..3230
                     /label=SV40\early\Promoter/Enhancer
     misc_feature    3129..3264
                     /label=SV40\origin
     misc_feature    3313..4107
                     /label=Kan(R)/Neo(R)
     misc_feature    4343..4361
                     /label=HSV\TK\polyA\signals
     misc_feature    4692..5335
                     /label=pUC\origin
BASE COUNT     1326 a      1443 c      1415 g      1233 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 
      721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 
      781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 
      841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 
      901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 
      961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 
     1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 
     1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 
     1141 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 
     1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga 
     1261 gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg 
     1321 tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc 
     1381 tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc accctggtga 
     1441 ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg 
     1501 acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg 
     1561 atgacggcaa ctacaagtcg cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc 
     1621 gcatcgagct gaccggcacc gatttcaagg aggatggcaa catcctgggc aataagatgg 
     1681 agtacaacta caacgcccac aatgtgtaca tcatgaccga caaggccaag aatggcatca 
     1741 aggtgaactt caagatccgc cacaacatcg aggatggcag cgtgcagctg gccgaccact 
     1801 accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt 
     1861 ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacttcg 
     1921 gcttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag tccggactca 
     1981 gatctcgagc tgatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag 
     2041 atccaaaaaa gaagagaaag gtaggatcca ccggatctag atagcggccg cgactctaga 
     2101 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 
     2161 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 
     2221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 
     2281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta 
     2341 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 
     2401 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 
     2461 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 
     2521 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 
     2581 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 
     2641 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 
     2701 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 
     2761 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 
     2821 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
     2881 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc 
     2941 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 
     3001 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 
     3061 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 
     3121 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 
     3181 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 
     3241 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga 
     3301 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 
     3361 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 
     3421 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 
     3481 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 
     3541 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 
     3601 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 
     3661 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 
     3721 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 
     3781 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 
     3841 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 
     3901 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 
     3961 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 
     4021 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 
     4081 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 
     4141 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 
     4201 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 
     4261 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg 
     4321 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt 
     4381 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac 
     4441 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg 
     4501 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt 
     4561 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     4621 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     4681 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     4741 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     4801 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     4861 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     4921 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     4981 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     5041 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     5101 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     5161 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     5221 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     5281 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     5341 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     5401 gtattaccgc catgcat 
//
