LOCUS       pDsRed-Monomer-F Hyg   5823 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632514
FEATURES             Location/Qualifiers
     CDS             613..1287
                     /label=DsRed-Monomer
     misc_feature    1303..1365
                     /label=c-Ha-Ras\farnesylation\signal
     promoter        1..589
                     /label=CMV\IE\Promoter
     rep_origin      complement(1680..2135)
                     /label=f1\origin
     rep_origin      2476..2614
                     /label=SV40\origin
     rep_origin      5098..5741
                     /label=pUC\origin
     CDS             2633..3658
                     /label=Hyg(R)
     CDS             4075..4935
                     /label=Amp(R)
     polyA_signal    1583..1588
                     /label=SV40\early\polyA\signal
     polyA_signal    1612..1617
                     /label=SV40\early\polyA\signal
     polyA_signal    3806..3811
                     /label=SV40\early\polyA\signal
     polyA_signal    3835..3840
                     /label=SV40\early\polyA\signal
     misc_feature    2532..2538
                     /label=SV40\early\promoter
     misc_feature    606..616
                     /label=Kozak\sequence
     promoter        4005..4033
                     /label=Amp(R)\Promoter
BASE COUNT     1455 a      1501 c      1492 g      1375 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg 
      661 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag 
      721 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc 
      781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc 
      841 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc 
      901 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc 
      961 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg 
     1021 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg 
     1081 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac 
     1141 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac 
     1201 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac 
     1261 gccgaggccc gccactccgg ctcccagtcc ggactcagat ctaagctgaa ccctcctgat 
     1321 gagagtggcc ccggctgcat gagctgcaag tgtgtgctct cctgaggatc cagatctcga 
     1381 gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga 
     1441 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 
     1501 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 
     1561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     1621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 
     1681 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 
     1741 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 
     1801 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 
     1861 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 
     1921 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 
     1981 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 
     2041 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 
     2101 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 
     2161 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     2221 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 
     2281 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 
     2341 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 
     2401 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 
     2461 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     2521 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     2581 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atatgaaaaa 
     2641 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc 
     2701 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg 
     2761 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt 
     2821 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt 
     2881 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct 
     2941 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc 
     3001 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca 
     3061 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca 
     3121 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct 
     3181 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa 
     3241 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg 
     3301 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga 
     3361 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg 
     3421 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt 
     3481 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac 
     3541 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga 
     3601 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag 
     3661 ttctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 
     3721 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 
     3781 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     3841 gcattttttt cactgcctcg cgatccacaa ccatggcgaa agggcctcgt gatacgccta 
     3901 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 
     3961 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     4021 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
     4081 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
     4141 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
     4201 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 
     4261 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt 
     4321 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
     4381 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
     4441 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
     4501 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
     4561 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
     4621 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
     4681 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
     4741 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
     4801 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
     4861 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
     4921 attaagcatt ggtaaggcgc gccaggccct gccatagcct caggttactc atatatactt 
     4981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     5041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     5101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     5161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     5221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     5281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     5341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     5401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     5461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     5521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     5581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     5641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     5701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     5761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 
     5821 cat 
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