LOCUS       pAcGFP1-N1   4726 bp    DNA   circular            13-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632469,632426,632485
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     misc_feature    591..671
                     /label=MCS
     CDS             2622..3416
                     /label=Kan(R)/Neo(R)
     rep_origin      4001..4644
                     /label=pUC\origin
     CDS             673..1389
                     /label=AcGFP1
     rep_origin      complement(1642..2097)
                     /label=f1\origin
     rep_origin      2438..2576
                     /label=SV40\origin
     polyA_signal    1545..1550
                     /label=SV40\early\polyA\signal
     polyA_signal    1574..1579
                     /label=SV40\early\polyA\signal
     polyA_signal    3652..3657
                     /label=HSV\TK\polyA\signal
     polyA_signal    3665..3670
                     /label=HSV\TK\polyA\signal
     misc_feature    2271..2500
                     /label=SV40\early\Promoter/Enhancer
     promoter        2159..2187
                     /label=Kan(R)\Promoter
BASE COUNT     1138 a      1267 c      1240 g      1081 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 
      721 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 
      781 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 
      841 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 
      901 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 
      961 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 
     1021 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 
     1081 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 
     1141 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 
     1201 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 
     1261 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 
     1321 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 
     1381 ctgtacaagt gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 
     1441 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 
     1501 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     1561 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     1621 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
     1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
     1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
     1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
     1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
     1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
     1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
     2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 
     2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 
     2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     2581 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 
     2641 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 
     2701 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 
     2761 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 
     2821 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 
     2881 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 
     2941 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 
     3001 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 
     3061 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 
     3121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 
     3181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 
     3241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 
     3301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 
     3361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 
     3421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 
     3481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     3541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 
     3601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 
     3661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 
     3721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 
     3781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 
     3841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 
     3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
     3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     4141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 
     4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 
     4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat 
//
