December
December 23, 2021
Wild birds like Mallard ducks are major reservoirs of avian influenza viruses responsible for global bird flu outbreaks. A recent paper reports high-quality reference genomes of the Mallard and two domesticated duck breeds, representing the most accurate and complete duck genome assembly to date. Their research serves as a rich resource for understanding the genetic basis of traits like egg and meat production, eggshell biomineralization, and provides important insights into avian evolution and the transmission of bird flu and other zoonotic viruses. Read now »
December 16, 2021
People living with HIV are at greater risk for age-related morbidities, mainly due to immune dysfunction with generalized T-cell and natural killer (NK) cell exhaustion. T‐cell immunoreceptor with immunoglobulin (Ig) and intracellular tyrosine inhibitory motif domains (TIGIT) negatively regulates effector function by differential expression in immune cell subsets. In this study, researchers tested whether alterations in NK cell TIGIT expression may be an effective strategy to invigorate antibody‐dependent NK cell activity against HIV‐1 infection. Read now »
December 9, 2021
Acute myeloid and lymphoid leukemias often contain chromosomal translocations related to the KMT2A gene. In this study, researchers mapped fusion-specific targets across the genome for diverse KMT2A oncofusion proteins in cell lines and patient samples. Single-cell CUT&Tag was performed using the ICELL8 system to investigate heterogeneity in single cells. The resulting patterns in their findings suggest that KMT2A fusion proteins activate distinct oncogenic networks within different cells of the same tumor, helping to characterize the global chromatin landscape of tumors. Read now »
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December 2, 2021
Researchers from Captis Diagnostics in collaboration with Carnegie Mellon University developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay was found to significantly reduce the running time of conventional RT-qPCR. Read now »
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November
November 18, 2021
Learn about how researchers from Eisai, Inc. (formerly Morphotek, Inc.) used the In-Fusion seamless cloning kit to help develop a robust high-throughput mAb discovery and production system. Their system integrates the advantages of high-efficiency cloning and stable pool selection methods, dramatically reducing the time and effort required to clone and express large numbers of amplified V genes—from 4–6 months to 4–6 weeks! Read now »
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November 11, 2021
The 2014 Ebola outbreak in West Africa resulted in more than 11,000 deaths, highlighting the need for a vaccine. Researchers from the Beijing Center for Disease Prevention and Control generated a recombinant Newcastle disease virus-based vaccine as a candidate for a heterologous prime-boost regimen against Ebola infection. Read now »
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November 4, 2021
Researchers at University Medical Center Göttingen utilized the SMART-Seq ICELL8 cx application to study the effect of the homozygous p.Ile221Thr mutation in YRDC. scRNA-seq was performed on patient fibroblasts to study changes in gene expression at the cellular level. Together with other functional analyses, researchers suggest that the biallelic mutant YRDC is linked to developmental disorders like segmental progeroid syndromes, and could result in defective tRNA modification, telomere shortening, and increased genomic instability. Read now »
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October
October 28, 2021
Researchers at City of Hope National Medical Center employed single cell transcriptomic profiling (scRNA-seq) performed on the ICELL8 system to examine the evolutionary trajectories of early stage breast cancer tumors using serial tumor biopsies from a clinical trial of preoperative endocrine therapy (letrozole) alone or in combination with the cell cycle inhibitor ribociclib. Their findings could potentially help optimize dosing and timing of combination therapies to counter treatment resistance in ER+ breast cancer. Read now »
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October 21, 2021
SARS-CoV-2 infection produces varying degrees of illness. One serious response is multisystem inflammatory syndrome in children (MIS-C), a severe, uncontrolled inflammatory response involving multiple organs. Researchers at Cedars-Sinai Medical Center used various approaches including RNA-seq and BCR repertoire analysis to identify which factors lead to severe prognosis. The authors identified a strong autoimmune signature in MIS-C involving multiple pathogenic pathways. Read now »
October 14, 2021
Researchers at the Helmholtz Pioneer Campus developed snRNA-seq2, an improved single-nucleus RNA-seq (snRNA-seq) method for nuclear transcriptome analyses from frozen, archived liver samples. Using this new method, the authors characterize the transcriptomes of individual hepatocytes with different levels of ploidy. They demonstrate that while hepatocytes in young mouse livers express stem cell markers regardless of ploidy, hepatocytes with different levels of ploidy adjust their gene dosage according to their position in the liver. Read now »
October 7, 2021
Researchers from Fred Hutchinson Cancer Research Center comprehensively characterized escape, breadth and potency across a panel of SARS-CoV-2 antibodies and identified neutralizing antibodies with exceptional sarbecovirus breadth and resistance to SARS-CoV-2 escape. Read now »
