Gene editing promotion
Save 50% on our Guide-it CRISPR Genome-Wide sgRNA Library System*
The Guide-it CRISPR Genome-Wide sgRNA Library System makes it easy to perform lentiviral-based, genome-wide phenotypic knockout screens using CRISPR/Cas9 in human cell lines. Each screen contains both the genome-wide sgRNA library and a Cas9 expression system in our easy-to-use Lenti-X Single Shots format, to produce cell lines stably expressing both Cas9 and the sgRNA library. Click here for everything you need to know about phenotypic screens using a CRISPR/Cas9 library.
- Contains >76,000 sgRNAs based on the Brunello library, which maximizes on-target specificity and activity while minimizing off-target effects (download sgRNA representation data)
- Each gene in the human genome is targeted by four highly active sgRNAs, enabling screens with a single module
- Modified sgRNA scaffold design for improved Cas9-sgRNA interaction and high editing efficiency
- Lentiviral sgRNA library is already formulated with the Lenti-X Packaging Single Shots and the Xfect Transfection Reagent, such that only water is required to produce a highly efficient transfection mix for transfecting Lenti-X 293T cells to produce high-titer sgRNA libraries
*Cite Discount Quote 32217 when ordering to receive the discount. Offer valid in the U.S. and Canada only through 4.30.19. Discount applies only to the product listed. No other discounts apply.
|Cat. #||Product||Size||List price||Sale price|
|632646||Guide-it CRISPR Genome-Wide sgRNA Library System||5 screens||
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