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Molecular Med Tri-Conference (MMTC)
The annual Molecular Medicine Tri-Conference (MMTC) is one of the world's leading international events in the fields of drug discovery, development, and diagnostics. The conference unites an ecosystem of 3,700 innovative thinkers and thought leaders throughout biotech, pharma, and academia from around the world. In 2019, it was held at the Moscone South Convention Center in San Francisco, California, from March 10–15.
We're looking forward to seeing you at MMTC 2020. In the meantime, we invite you to review our materials from past conferences and reach out to us with any questions or requests via the "speak with us" link below.
MMTC 2019: poster
Next-generation whole genome amplification methods for CNV and SNV detection from single cells
Preparation of amplified genomic material from small amounts of DNA or single cells is an essential research tool in assisting genetic analyses of clinical samples aimed at identifying the best treatment regimen and molecular diagnoses of diseases such as cancer. Technologies that allow for accurate, reproducible detection of single nucleotide variation (SNV) and copy number variation (CNV) of genomic material from limited samples need to do so with high fidelity and high genome coverage. Additionally, they should be flexible enough to be used in a variety of analysis platforms. To address these research needs, we have developed the PicoPLEX WGA V2 System, a platform-agnostic whole genome amplification system, and the PicoPLEX Gold Single Cell DNA-Seq Kit, a complete cells-to-library solution for Illumina sequencers. These systems use optimized enzymes, primers, and protocols for optimal sequencing coverage, uniformity, and accuracy to detect SNVs, all the while increasing the resolution for CNV detection relative to previous versions. Both systems maintain the technology's simple workflow and unmatched cell-to-cell reproducibility that is a hallmark of the PicoPLEX technology. In this study, we have demonstrated CNV detection to 5-mb resolution at a depth of 1 million read pairs in a single run, with validated copy-number gains and losses. SNV detection and reproducibility are shown to be superior to competitive technologies.
A novel protocol that enables the detection of both SNVs and CNVs from 1-5 cells in a single, low-pass sequencing run that saves time and money.
Takara Bio USA, Inc.
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