- Speak with us
The annual LakePharma Symposium brings together individuals and organizations in the biotech community to discuss the latest developments in biologics. Takara Bio enjoyed meeting colleagues who attended the 2019 LakePharma Symposium in South San Francisco, CA.
We're looking forward to seeing you at next year's symposium. In the meantime, we invite you to learn more about Takara Bio's interest in biologics research and reach out to us with any questions or requests via the "speak with us" link below.
LakePharma Symposium 2019: poster and featured technology
Rapid conjugation, proteolysis, and purification of antibodies using high-capacity Capturem membranes
Capturem is a new technology that consists of a porous, high-capacity membrane that can be functionalized with not only proteases (e.g., trypsin or pepsin) but also with other ligands such as Protein A or G, nickel, streptavidin, etc. The functionalized membranes are assembled in different spin column formats and multiwell plates to allow for rapid purification, enrichment, labeling, or spin digest. Here we show the digestion of antibodies within ~2–3 minutes using Capturem Trypsin 96-well plates, and a 15-minute protocol for antibody conjugation and purification using Protein G Miniprep Columns without the need to purify before and after labeling. This allows the fast digestion and analysis of protein and antibody samples in a high-throughput fashion.
Featured technology: Capturem Trypsin
Capturem Trypsin combines the power of trypsin digest with our high-capacity Capturem membrane technology for the complete digestion of protein samples in as little as 3–6 minutes. The high local trypsin concentration plus a large internal surface area across a short distance equals almost instant binding and cleavage at room temperature. Digestion with Capturem Trypsin yields specific peptide fragments suitable for mass spectrometry analysis and is available in a 96-well format for high-throughput processing with excellent well-to-well reproducibility.
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