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View the data for Capturem EV isolation View data on Capturem EV isolation

International Society for Extracellular Vesicles

extracellular vesicle isolation

ISEV unites experts worldwide studying extracellular vesicles, exosomes, and microvesicles. With the mission to advance global extracellular research, ISEV hosts annual meetings, workshops, and other events throughout the year.

Takara Bio attended the first Joint Conference on Extracellular Vesicles in Cancer, on August 2–4, 2019, at Vanderbilt University in Nashville, TN. Cohosted by the ISEV along with the Metastasis Research Society (MRS), this conference brought together leaders in extracellular vesicle (EV) biology and cancer biology. We were excited to attend this first-ever meeting and be among those sharing the latest research in understanding the contributions of EVs to cancer progression.

We invite you to review the materials we presented and reach out to us with any questions or requests via the "speak with us" link below.

Learn more about ISEV »

Joint Conference on Extracellular Vesicles in Cancer 2019: poster and featured technology

scientific poster Use of high-capacity membranes for simple, rapid extracellular vesicle isolations with high yield and purity

Poster thumbnail

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Despite their small size, extracellular vesicles (EV) play important roles in normal physiological processes (e.g., immune response, neuronal function, and stem cell maintenance) and diseases (e.g., cancer and liver disease). A key bottleneck in EV research is the isolation of the vesicles, which has historically been accomplished via ultracentrifugation. However, ultracentrifugation is time-consuming, is not scalable, requires specialized equipment, may damage vesicles during the high-speed spins, and suffers from low yield. More recently, precipitation solutions have been utilized to simplify EV isolation protocols, but these techniques are often inconsistent, with low yield and reduced purity. Thus, there is a significant need for a method to rapidly isolate EVs without compromising purity or yield.

Here we describe the use of novel membranes conjugated to a proprietary, nonantibody-based EV-binding compound to selectively isolate EVs. The membranes, which we have named Capturem membranes, have been chemically modified to have increased surface area, which allows higher binding capacity while providing highly pure and concentrated samples. Additionally, the membranes have been assembled into benchtop centrifuge-compatible spin columns which can be used to isolate EVs in under 30 minutes.

Isolations performed with the Capturem EV isolation spin columns produced EVs of sizes comparable to experimental values reported in the literature; contained the key EV protein markers CD63, CD9, and Alix; and showed little or no expression of the EV-negative markers calnexin and albumin. Furthermore, we were able to collect ~3 ng total RNA from exosomes isolated from a single spin column (using an input of as little as 50 µl of plasma), enough total RNA to generate multiple cDNA libraries for downstream NGS analysis. These Capturem columns enable researchers studying EVs to accelerate the pace of their research by obtaining high yields of noncontaminated exosomes in a simple and rapid manner.

Capturem EV Isolation technology overview Featured technology: Capturem Extracellular Vesicle Isolation Kit (Mini)

Capturem EV isolation miniprep workflow

Learn more »

Rapid, pure, and concentrated purification of extracellular vesicles

The Capturem Extracellular Vesicle Isolation Kit (Mini) consists of spinnable affinity columns containing novel, modified nylon membranes conjugated to a lectin-based, EV-binding compound, thus providing a unique solution for the isolation of concentrated, highly pure EVs. This system removes the bottleneck in EV research presented by traditional ultracentrifugation-based EV isolation methods, which suffer from low yield, reduced purity, and time-consuming protocols. With a room-temperature protocol that takes less than 30 minutes, the Capturem EV isolation kit can rapidly enrich EVs from plasma and other biofluids for downstream proteomic, genomic, and transcriptomic analyses.


Rapid, pure, and concentrated purification of extracellular vesicles from biofluids

View data on how to consistently obtain purely concentrated EVs with enough yield for subsequent proteomic, genomic, and transcriptomic analyses.

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Highlighted conferences

Next Generation Dx Summit

The Next Generation Dx Summit brings together research and industry experts to discuss advancements in diagnostics technology across a range of topics.

Single Cell Genomics

The Single Cell Genomics conference brings together research and industry experts to discuss advances in single-cell genomics technologies.

CSHL Genome Engineering

CSHL Genome Engineering conference fosters creative interactions between researchers working on the basic biology of CRISPR/Cas and those applying this technology.

ASRM Scientific Congress

ASRM brings together research and industry experts to discuss advances in reproductive medicine.

PharmSci 360

The AAPS PharmSci 360 annual meeting brings experts from research and industry together to discuss all aspects of the pharmaceutical sciences.

AMP Annual Meeting & Expo

The AMP Annual Meeting & Expo brings together the international molecular diagnostics community.

Oxford Global NGS Congress

The Oxford Global NGS general meeting brings together research and industry experts to discuss advances in next-generation sequencing technologies.

SLAS Conference

SLAS brings together experts who promote the synthesis of new technologies, partnerships, and ideas that advance laboratory automation and screening workflows.

World Preclinical Congress

WPC brings together research, clinical, and industry experts to discuss the state and advances of preclinical research and technologies.

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Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis. This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. Capturem Trypsin Columns may be used to completely digest protein samples in less than a minute with digestion efficiencies (protein coverage) comparable to or better than those obtained using in-solution trypsin digestion.

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