American Society for Mass Spectrometry (ASMS) annual conference
The American Society for Mass Spectrometry (ASMS) annual conference brings together research and industry experts to discuss advancements in mass spectrometry technologies. Our trypsin and pepsin digestion products offer support for mass spec sample preparation, a key step which often requires the conversion of proteins to peptides.
We're looking forward to seeing you at ASMS 2020 and invite you to review the materials we presented at past meetings. You can also reach out to us with any questions or requests via the "speak with us" link below.
ASMS 2019: poster and featured technology
Rapid conjugation, proteolysis, and purification of antibodies using high-capacity Capturem membranes
Capturem is a new technology that consists of a porous, high-capacity membrane that can be functionalized with not only proteases (e.g., trypsin or pepsin) but also with other ligands such as Protein A or G, nickel, streptavidin, etc. The functionalized membranes are assembled in different spin column formats and multiwell plates to allow for rapid purification, enrichment, labeling, or spin digest. Here we show the digestion of antibodies within ~2–3 minutes using Capturem Trypsin 96-well plates, and a 15-minute protocol for antibody conjugation and purification using Protein G Miniprep Columns without the need to purify before and after labeling. This allows the fast digestion and analysis of protein and antibody samples in a high-throughput fashion.
Featured technology: Capturem Trypsin
Capturem Trypsin combines the power of trypsin digest with our high-capacity Capturem membrane technology for the complete digestion of protein samples in as little as 3–6 minutes. The high local trypsin concentration plus a large internal surface area across a short distance equals almost instant binding and cleavage at room temperature. Digestion with Capturem Trypsin yields specific peptide fragments suitable for mass spectrometry analysis and is available in a 96-well format for high-throughput processing with excellent well-to-well reproducibility.
Protein digestion resources
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